The effects of exposure at room temperature to pharmacopuncture within a syringe: an investigation of changes in microbiological safety

Chong Hwan Lee; Jin Ho Lee; In Hyuk Ha; Me Riong Kim; In Hee Lee; Jae Woong Lee; Eun Jee Kim; Hae Sol Kim; Ho Sun Kim; Young Hyeon Bae; No Hyeon Kim; Chang Yong Suh; Jang Hoon Byun; Sang Won Park; Min Jeong Kim

doi:10.13045/acupunct.2015060

J Acupunct Res > Volume 32(4); 2015 > Article

Lee, Lee, Ha, Kim, Lee, Lee, Kim, Kim, Kim, Bae, Kim, Suh, Byun, Park, and Kim: The effects of exposure at room temperature to pharmacopuncture within a syringe: an investigation of changes in microbiological safety※

Original Article

Acupuncture 2015; 32(4): 37-45.

DOI: https://doi.org/10.13045/acupunct.2015060

The effects of exposure at room temperature to pharmacopuncture within a syringe: an investigation of changes in microbiological safety^※

Chong Hwan Lee¹, Jin Ho Lee^2,³, In Hyuk Ha^2,³, Me Riong Kim^2,³, In Hee Lee², Jae Woong Lee², Eun Jee Kim², Hae Sol Kim¹, Ho Sun Kim¹, Young Hyeon Bae¹, No Hyeon Kim¹, Chang Yong Suh¹, Jang Hoon Byun³, Sang Won Park³, Min Jeong Kim^2,^*

¹Department of Acupuncture and Moxibustion Medicine, Gang-Nam Jaseng Oriental Medicine Hospital

²Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation

³Department of Rehabilitation Medicine of Korean Medicine, Gang-Nam Jaseng Oriental Medicine Hospital

^*Corresponding author: Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation, 3F, 858, Eonju-ro, Gangnam-gu, Seoul, 135-896, Republic of Korea,
Tel: +82-2-3218-2251, E-mail: lkomvje@jaseng.co.kr

^※ This study was supportd by Korea Promotion Institute for Traditional Medicine Industry(KOTMIN) service task. Thanks for funding.

Received November 19, 2015 Revised December 01, 2015 Accepted December 02, 2015

See the Original "".

Abstract

Objectives:

The main aim of this study is to evaluate changes in the microbiological safety of pharmacopuncture exposed to room temperature for an elapsed period of time.

Methods:

The four most frequently used pharmacopuncture products were stored in syringes at room temperature at three different hospitals for 24 hours and 48 hours respectively and they were compared with pharmacopuncture products stored in vials through a sterility and microbial limited test.

Results:

Storage forms and duration of exposure to room temperature did not show significant difference in bacterial or fungal contamination which was confirmed by the sterility and microbial limited test.

Conclusions:

Pharmacopuncture products stored in syringes at room temperature for 24 hours and 48 hours demonstrated their safety in terms of lack of microbiological contamination.

Key Words: Pharmacopuncture; Safety; Microbiological safety.

Fig. 1

Sterility test result of Shinbaro pharmacopuncture

Fig. 2

Sterility test result of Hwangryun-Hae-Dok-tang pharmacopuncture

Fig. 3

Sterility test result of Jungsongouhyul pharmacopuncture

Fig. 4

Sterility test result of Bee venom pharmacopuncture

Fig. 5

Limited test(Bacteria) result of Shinbaro pharmacopuncture

Fig. 6

Limited test(Fungi) result of Shinbaro pharmacopuncture

Fig. 7

Limited test(Bacteria) result of Hwangryun-Hae-Dok-tang pharmacopuncture

Fig. 8

Limited test(Fungi) result of Hwangryun-Hae-Dok-tang pharmacopuncture

Fig. 9

Limited test(Bacteria) result of Jung-songouhyul pharmacopuncture

Fig. 10

Limited test(Fungi) result of Jungsongouhyul pharmacopuncture

Fig. 11

Limited test(Bacteria) result of Bee venom pharmacopuncture

Fig. 12

Limited test(Fungi) result of Bee venom pharmacopuncture

Table 1

Strain and Criteria for Media Probability Test

Strain	used medium	Criteria
Clostridium sporogenes Pseudomonas aeruginosa Staphylococcus aureus	Thioglycollate medium	Certain Growth of Strain
Aspergillus brasiliensis Bacillus subtilis Candida albicans	Tryptic soy agar	Certain Growth of Strain

Table 2

Strain and Criteria for Validation Test

Strain	used medium	Criteria
Clostridium sporogenes Pseudomonas aeruginosa Staphylococcus aureus	Thioglycollate medium	Same Growth between Control group
Aspergillus brasiliensis Bacillus subtilis Candida albicans	Tryptic soy agar	Same Growth between Control group

Table 3

Test Result

		Sterility test			Limited test
		0 h	24 h	48 h	0 h	24 h	48 h
Dae jeon	SBO	–	–	–	–	–	–
	HR	–	–	–	–	–	–
	JS	–	–	–	–	–	–
	BV	–	–	–	–	–	–
Bu cheon	SBO	–	–	–	–	–	–
	HR	–	–	–	–	–	–
	JS	–	–	–	–	–	–
	BV	–	–	–	–	–	–
Hae un dae	SBO	–	–	–	–	–	–
	HR	–	–	–	–	–	–
	JS	–	–	–	–	–	–
	BV	–	–	–	–	–	–

VI.

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