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J Acupunct Res > Volume 32(2); 2015 > Article
Yang, Kim, Kim, Park, Yang, Choi, and Wei: The Effects of Sophorae Radix Pharmacopuncture Therapy on the Chunchu(ST25) for DSS-induced Colitis in Rats
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Abstract

Objectives:

The purpose of this study is to investigate the effects of Sophorae radix pharmacopuncture(SRP) therapy on the Chunchu(ST25) on intestinal disease in rats with dextran sulfate sodium(DSS)-induced colitis.

Methods:

The experimental model of colitis was induced by an infection of DSS for fifteen days. After colitis was induced, SRP therapy was practiced on the Chunchu(ST25) once every three days for a total of five times. Afterward, colon length, damage to the colonic mucosa, complete blood count, aspartate aminotransferase(AST), alanine transferase(ALT), blood urea nitrogen(BUN), creatinine, TNF-α and IL-6 were measured.

Results:

The results were obtained as below.
  1. Colon length was significantly increased in the SRP group 2 compared to the control group.

  2. Damage of colonic mucosa was observed less in the SRP groups than in the other groups.

  3. The mean rates of white blood cell(WBC), neutrophils and lymphocytes were significantly increased in SRP group 2 compared to the control group.

  4. The mean rates of HGB and hematocrit(HCT) were significantly increased in the SRP compared to the control group.

  5. No significant differences were shown in AST, ALT, BUN and Creatinine between the SRP groups and the control group.

  6. The mean rate of TNF-α was significantly decreased in the SRP groups compared to the control group.

  7. The mean of IL-6 was significantly decreased in the SRP groups compared to the control group.

Conclusions:

These results indicated that SRP therapy on Chunchu(ST25) functions as a treatment to the dextran sulfate sodium-induced colitis.

Fig. 1.
Effects of Sophorae radix pharmacopuncture on colon length in DSS- induced colitis
Experimental colitis in rat was induced by a 5 % DSS dissolved in the drinking water for 15 days. DSS was administered water for 15 days prior to 5 % DSS supplement. The colon lengths were measured. The groups refer to table 1.
Values are expressed mean±SE.
**: p<0.01 compared with control.
acupunct-32-2-77f1.gif
Fig. 2.
Effectds of Sophorae radix pharmacopuncture chronic colitis model induced by DSS
Effect of acupoint on colon injury. Representative photomicrograph colonic mucosa with haematoxylin and eosin from a rat.
A: no therapy with 5 % DSS. B: distilled water and no therapy with 5 % DSS.
C: 5 % DSS and Sophorae radix pharmacopuncture 0.2×.
D: 5 % DSS and Sophorae radix pharmacopuncture 1×.
acupunct-32-2-77f2.gif
Fig. 3.
Effects of Sophorae radix pharmacopuncture on the level of TNF-a in DSS-treated rats plasma
The groups refer to table 1.
Values are expressed mean±SE.
**: p<0.01 compared with control.
acupunct-32-2-77f3.gif
Fig. 4.
Effects of Sophorae radix pharmacopuncture on the level of IL-6 in DSS-treated rat plasma
Values are expressed mean±SE.
**: p<0.01 compared with control.
acupunct-32-2-77f4.gif
Table 1.
Changes on the Blood WBC, Neutrophils, Lymphocytes and Monocytes Contents after Sophorae Radix Pharmacopuncture Treatments in DSS Induced Colitis in Rats
Group WBC(K/uL) Neutrophils(K/uL) Lymphocytes(K/uL) Monocytes(K/uL) Eosinophils(K/uL)
Control 3.79±0.30 0.81±0.06 2.85±0.23 0.12±0.02 0.010±0.005
Saline 3.36±0.53 0.91±0.20 2.32±0.31 0.12±0.03 0.016±0.004
SRP 1 3.78±0.64 0.91±0.12 2.68±0.49 0.18±0.03 0.006±0.004
SRP 2 5.71±0.52** 1.26±0.17* 4.19±0.38** 0.21±0.06 0.004±0.002

The groups refer to table 1. Values are expressed mean±SE.

* p<0.05,

** p<0.01 compared with control.

Table 2.
Changes on the Blood RBC, HGB, HCT, MCV, MCH, and MCHC Contents after Sophorae Radix Pharmacopuncture Treatments in DSS Induced Colitis in Rats
Group RBC(K/uL) HGB(g/dL) HCT(%) MCV(fL) MCH(pg) MCHC(g/dL)
Control 7.41±0.24 13.18±0.12 52.08±1.09 70.47±0.97 17.88±0.48 25.35±0.36
Saline 7.08±0.23 12.54±0.42 49.40±1.54 69.78±0.50 17.68±0.09 25.38±0.17
SRP 1 7.52±0.12 13.34±0.06 52.52±0.48 69.90±1.24 17.76±0.33 25.40±0.30
SRP 2 7.79±0.12 14.28±0.3** 56.08±0.9* 71.98±0.80 18.34±0.15 25.42±0.30

The groups refer to table 1. Values are expressed mean±SE.

* p<0.05,

** p<0.01 compared with control.

Table 3.
Changes on the AST, ALT, BUN, CRE after Sophorae Radix Pharmacopuncture treatments in DSS Induced Colitis in Rats
Group AST(U/I) ALT(U/I) BUN(mg/dl) CRE(mg/dl)
Control 99.0±6.76 37.8±2.04 13.7±0.42 0.2±0.02
Saline 118.4±24.7 39.2±4.75 17.5±1.70 0.3±0.02
SRP 1 97.8±15.07 45.2±10.1 14.5±1.08 0.2±0.02
SRP 2 99.0±8.51 37.2±2.08 16.1±0.97 0.2±0.00

The groups refer to table 1. Values are expressed mean±SE.

VI.
VI.

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