The Effects of Eucommiae Cortex Pharmacopuncture Injected at Sinsu(BL23) on Neuropathic Pain in Rats

Article information

J Korean Acupunct Moxib Soc. 2013;30(4):69-78
Publication date (electronic) : 2013 September 20
doi : https://doi.org/10.13045/acupunct.2013023
Department of Acupuncture and Moxibustion Medicine, College of Oriental Medicine, Dongshin University
*Corresponding author : Department of Acupuncture & Moxibustion Medicine, Gwangju Oriental Medicine Hospital of Dongshin University, 377-12, Wolsan-dong, Nam-gu, Gwangju, 503-850, Republic of Korea, Tel : +82-62-350-7209 E-mail : nahonga@hanmail.net
Received 2013 August 06; Revised 2013 August 15; Accepted 2013 August 26.

Abstract

Objectives :

The purpose of this study is to examine whether Eucommiae Cortex pharmacopuncture may affect to the neuropathic pain in a rat model.

Methods :

To produce the model of neuropathic pain, under isoflurane 2.5 % anesthesia, underwent tight ligation by 6.0 silk thread and transection of the tibial and sural nerves, leaving the common peroneal nerve intact. After neuropathic surgery, the author examined if the exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the plantar with Dynamic Plantar Aesthesiometer. Three days after the neuropathic surgery, Eucommiae Cortex pharmacopuncture was injected at Sinsu(BL23) once every week for 6 weeks. After that, the author examined the withdrawal response of neuropathic rats’ leg by Dynamic Plantar Aesthesiometer.

And also the author examined Bax, Bcl-2, Bax/Bcl-2 ratio in the spinal cord of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats.

Results :

  1. The Eucommiae Cortex pharmacopuncture decreased the withdrawal response of mechanical allodynia that assessed with Dynamic Plantar Aesthesiometer in EC2-BL23 group as compared with control group.

  2. The Eucommiae Cortex pharmacopuncture decreased Bax/Bcl-2 ratio in EC1-BL23, EC2-BL23 group. But The Eucommiae Cortex pharmacopuncture injected at Sinsu(BL23) didn’t change Bax, Bcl-2 expression level in the all group.

  3. The Eucommiae Cortex pharmacopuncture decreased WBC count in EC1-BL23, EC2-BL23 group.

Conclusions :

We have noticed that Eucommiae Cortex pharmacopuncture decreased mechanical allodynia in the model of neuropathic pain compared with the control group. Bax/Bcl-2 ratio in spinal cord of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of neuropathic pain.

Fig. 1.

Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal React Time in Neuropathic Pain Rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.

Fig. 2.

Effects of pharmacopuncuture(Eucommiae Cortex) at BL23 acupoint on the withdrawal force intensity in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.

Fig. 3.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the intensity of bax mRNA in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

Fig. 4.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the intensity of bcl-2 mRNA in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

Fig. 5.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the bax/bcl-2 ratio in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.

Fig. 6.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the WBC, RBC, HGB, HCT in hematology of neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, as compared with the corresponding data of control group.

Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal React Time in Neuropathic Pain Rats (%)

Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal Force Intensity in Neuropathic Pain Rats (%)

Effects of Pharmacopuncture(Eucommiae Cortex) at BL23 Acupoint on the WBC, RBC, HGB, HCT in Hematology of Neuropathic Pain Rats

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Article information Continued

Fig. 1.

Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal React Time in Neuropathic Pain Rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.

Fig. 2.

Effects of pharmacopuncuture(Eucommiae Cortex) at BL23 acupoint on the withdrawal force intensity in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.

Fig. 3.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the intensity of bax mRNA in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

Fig. 4.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the intensity of bcl-2 mRNA in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

Fig. 5.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the bax/bcl-2 ratio in neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, ** : p<0.001, as compared with the corresponding data of control group.

Fig. 6.

Effects of pharmacopuncture(Eucommiae Cortex) at BL23 acupoint on the WBC, RBC, HGB, HCT in hematology of neuropathic pain rats

Control : neuropathic pain induced and no treatment.

Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

* : p<0.05, as compared with the corresponding data of control group.

Table 1.

Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal React Time in Neuropathic Pain Rats (%)

Weeks 1 2 3 4 5 6
Group
  Control 100.±0.00 122.6±21.20 128.3±24.81 102.1±10.94 173.7±21.46 176.3±47.93
  Saline 100.±0.00 141.9±47.06 76.6±15.33 77.4±13.91 202.7±51.36 144.7±58.31
  EC-1 100.±0.00 95.6±24.42 110.2±42.84 69.5±14.32 139.6±40.45 131.4±45.71
  EC-2 100.±0.00 66.3±15.47 54.8±9.05* 34.8±7.00** 64.9±13.68* 65.5±10.02*

Pharmacopuncture was treated on the rats for 6 weeks.

Results are shown as means±SE.

*:

p<0.05,

**:

p<0.001, as compared with the corresponding data of control group.

Table 2.

Effects of Pharmacopuncuture(Eucommiae Cortex) at BL23 Acupoint on the Withdrawal Force Intensity in Neuropathic Pain Rats (%)

Weeks 1 2 3 4 5 6
Group
  Control 100.±0.00 122.0±20.69 127.5±24.27 102.0±10.83 160.0±22.94 169.6±43.80
  Saline 100.±0.00 130.9±36.52 77.1±15.08 77.8±13.69 169.8±29.53 122.5±34.39
  EC-1 100.±0.00 95.5±23.87 109.5±41.67 70.0±14.21 138.4±39.51 130.3±44.53
  EC-2 100.±0.00 66.9±15.14* 55.5±8.93* 35.9±6.86** 65.5±13.34* 66.1±9.80*

Pharmacopuncture was treated on the rats for 6 weeks.

Results are shown as means±SE.

*:

p<0.05,

**:

p<0.001 as compared with the corresponding data of control group.

Table 3.

Effects of Pharmacopuncture(Eucommiae Cortex) at BL23 Acupoint on the WBC, RBC, HGB, HCT in Hematology of Neuropathic Pain Rats

Group Control Saline EC-1 EC-2
Group
  WBC(K/ul) 6.5±0.52 5.9±0.41 4.7±0.48* 4.6±0.22*
  RBC(M/ul) 8.5±0.13 8.6±0.19 8.7±0.15 8.7±0.17
  HGB(g/dl) 15.4±0.33 14.8±0.45 16.0±0.35 14.9±0.22
  HCT(%) 55.1±1.04 53.9±2.31 55.5±1.62 53.2±0.46

Control : neuropathic pain induced and no treatment. Saline : neuropathic pain induced and saline injection.

EC-1 : neuropathic pain induced and 9.1 μg/kg/μl pharmacopuncture.

EC-2 : neuropathic pain induced and 45.5 μg/kg/μl pharmacopuncture.

Results are shown as mean±SE.

*:

p <0.05 as compared with the corresponding data of control group.